Characterization of the Nuclear Lamina in an Insect by Differential Staining

نویسنده

  • Ruth Marx
چکیده

Nuclear Lamina, Insect, Fat Body, Phosphotungstic Acid, Protein Staining The nuclear envelope of the larval fat body in the blow­ fly Calliphora vicina reveals a conspicuous nuclear lamina. It appears homogeneous, Fibrous or granular depending upon the fixing and staining methods. The best relative contrast for the nuclear lamina and the nuclear pore com­ plex is obtained with a phosphotungstic acid staining pro­ cedure designed for proteins.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Introducing a combined Leishman-Giemsa stain as a new staining technique for avian blood smears

Background: The use of conventional stains including Giemsa, Wright and Leishman have become an essential tool for differential diagnosis of cells in peripheral blood. Aims: The aim of the study was to develop a new combination of Leishman-Giemsa (L&G;) stain for avian blood smears and to compare its efficacy with conventional staining methods. Methods:<...

متن کامل

Reporting an Experience: Improving the Feulgen Staining Technique for Better Visualizing of Nucleus

Among different staining methods used to demonstrate the nuclear abnormalities, Feulgen is one of the most reliable method. Feulgen staining is specific, sensitive method for evaluating the DNA damages.It has been shown that using non-DNA specific stains for monitoring the nuclear anomalies lead to false-positive or false-negative results. From self-experience, immersing the stained slides in h...

متن کامل

Calretinin Immunohistochemistery: An Aid in the Diagnosis of Hirschsprung’s Disease

Objective(s) Definite diagnosis of Hirschsprung’s disease (HD) is based on histopathological study, but there are limitations associated with standard histology and histochemistry in this regard. The aim of this study was to investigate calretinin immunostaining patterns in both ganglionic and aganglionic HD intestinal specimens and to compare them with control specimens. Materials and Method...

متن کامل

Purification and characterization of antiviral protein from silkworm fecal matter

Antiviral proteins (AVP), present in silkworm fecal matter, show activity against nuclear polyhedrosis virus (NPV) in vitro and in vivo. The extract of silkworm fecal matter prepared in phosphate buffer solution of pH 7.5 was subjected to 50% solid ammonium sulfate precipitation to enrich AVP, then which was dialyzed. The dialysate was applied to the column containing silica gel-G, the column e...

متن کامل

Multiple Hypercementosis: Report of a Rare Presentation

Abstract  Hypercementosis is identified by an excessive, non-neoplastic deposition of radicular cementum and is mostly presented as a solitary lesion or in rare cases as a multiple type. It usually occurs in the premolar and molar region of mandible with no sex predilection. In this paper, we reported a 57-year-old female patient with multiple radiopaque lesions affecting right maxillary second...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2013